Review



cd81  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Proteintech cd81
    Cd81, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/CD81+Antibody/pmc13038339-74-45-50
    Average 96 stars, based on 514 article reviews
    cd81 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Ferroptosis-dependent small extracellular vesicles ULK1 enhances mitophagy and suppresses breast cancer migration.
    Article Snippet: .. Membranes were blocked in 5% non-fat milk prepared in TBST and then incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (abcam, ab216130, 1:1000 dilution), anti-ULK1 (Proteintech, 20986-1-AP, 1:20000 dilution), anti-CD81 (Proteintech, 27855-1-AP, 1:5000 dilution), anti-CD9 (Proteintech, 20597- 1-AP, 1:5000 dilution), anti-calnexin (Proteintech, 10427-2-AP, 1:10000 dilution), anti-LC3 (Proteintech, 14600-1-AP 1:4000 dilution), anti-TOM20 (Proteintech, 11802-1-AP, 1:20000 dilution), anti-CD86 (Santa cruz, sc-19617, 1:200 dilution), anti-206 (Proteintech, 18704-1-AP, 1:2000 dilution), and anti-GAPDH (Proteintech, 60004-1-Ig, 1:2000 dilution) as a loading control. .. After washing, membranes were incubated with appropriate HRP-conjugated secondary antibodies Goat Anti-Rabbit IgG H&L (Beyotime, A0208,1:1000 dilution) or Goat Anti-Mouse IgG H&L AR TIC LE IN PR ES S (Beyotime, A0216, 1:1000 dilution) for 1 hour at room temperature.

    Article Title: Ferroptosis-dependent small extracellular vesicles ULK1 enhances mitophagy and suppresses breast cancer migration
    Article Snippet: .. Membranes were blocked in 5% non-fat milk prepared in TBST and then incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (abcam, ab216130, 1:1000 dilution), anti-ULK1 (Proteintech, 20986-1-AP, 1:20000 dilution), anti-CD81 (Proteintech, 27855-1-AP, 1:5000 dilution), anti-CD9 (Proteintech, 20597-1-AP, 1:5000 dilution), anti-calnexin (Proteintech, 10427-2-AP, 1:10000 dilution), anti-LC3 (Proteintech, 14600-1-AP 1:4000 dilution), anti-TOM20 (Proteintech, 11802-1-AP, 1:20000 dilution), anti-CD86 (Santa cruz, sc-19617, 1:200 dilution), anti-206 (Proteintech, 18704-1-AP, 1:2000 dilution), and anti-GAPDH (Proteintech, 60004-1-Ig, 1:2000 dilution) as a loading control. .. After washing, membranes were incubated with appropriate HRP-conjugated secondary antibodies Goat Anti-Rabbit IgG H&L (Beyotime, A0208,1:1000 dilution) or Goat Anti-Mouse IgG H&L (Beyotime, A0216, 1:1000 dilution) for 1 h at room temperature.

    Control:

    Article Title: Ferroptosis-dependent small extracellular vesicles ULK1 enhances mitophagy and suppresses breast cancer migration.
    Article Snippet: .. Membranes were blocked in 5% non-fat milk prepared in TBST and then incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (abcam, ab216130, 1:1000 dilution), anti-ULK1 (Proteintech, 20986-1-AP, 1:20000 dilution), anti-CD81 (Proteintech, 27855-1-AP, 1:5000 dilution), anti-CD9 (Proteintech, 20597- 1-AP, 1:5000 dilution), anti-calnexin (Proteintech, 10427-2-AP, 1:10000 dilution), anti-LC3 (Proteintech, 14600-1-AP 1:4000 dilution), anti-TOM20 (Proteintech, 11802-1-AP, 1:20000 dilution), anti-CD86 (Santa cruz, sc-19617, 1:200 dilution), anti-206 (Proteintech, 18704-1-AP, 1:2000 dilution), and anti-GAPDH (Proteintech, 60004-1-Ig, 1:2000 dilution) as a loading control. .. After washing, membranes were incubated with appropriate HRP-conjugated secondary antibodies Goat Anti-Rabbit IgG H&L (Beyotime, A0208,1:1000 dilution) or Goat Anti-Mouse IgG H&L AR TIC LE IN PR ES S (Beyotime, A0216, 1:1000 dilution) for 1 hour at room temperature.

    Article Title: Ferroptosis-dependent small extracellular vesicles ULK1 enhances mitophagy and suppresses breast cancer migration
    Article Snippet: .. Membranes were blocked in 5% non-fat milk prepared in TBST and then incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (abcam, ab216130, 1:1000 dilution), anti-ULK1 (Proteintech, 20986-1-AP, 1:20000 dilution), anti-CD81 (Proteintech, 27855-1-AP, 1:5000 dilution), anti-CD9 (Proteintech, 20597-1-AP, 1:5000 dilution), anti-calnexin (Proteintech, 10427-2-AP, 1:10000 dilution), anti-LC3 (Proteintech, 14600-1-AP 1:4000 dilution), anti-TOM20 (Proteintech, 11802-1-AP, 1:20000 dilution), anti-CD86 (Santa cruz, sc-19617, 1:200 dilution), anti-206 (Proteintech, 18704-1-AP, 1:2000 dilution), and anti-GAPDH (Proteintech, 60004-1-Ig, 1:2000 dilution) as a loading control. .. After washing, membranes were incubated with appropriate HRP-conjugated secondary antibodies Goat Anti-Rabbit IgG H&L (Beyotime, A0208,1:1000 dilution) or Goat Anti-Mouse IgG H&L (Beyotime, A0216, 1:1000 dilution) for 1 h at room temperature.

    Blocking Assay:

    Article Title: Downregulated lysyl oxidase in plasma extracellular vesicles: a biomarker linked to brain metastasis risk in lung adenocarcinoma.
    Article Snippet: .. After blocking with 5% skim milk, the membrane was probed overnight at 4 °C with the following primary antibodies: anti-CD81 (66866-1-Ig, Proteintech), anti-CD9 (25597-1-AP, Proteintech), anti-TSG101 (ab125011, Abcam), anti-HSP70 (10995-1-AP, Proteintech) and anti-Calnexin (ab22595, Abcam). .. Subsequently, the membrane was washed and incubated with HRP-conjugated secondary antibodies (SA00001-1 and SA00001-2, Proteintech) for 1 h at room temperature.

    Membrane:

    Article Title: Downregulated lysyl oxidase in plasma extracellular vesicles: a biomarker linked to brain metastasis risk in lung adenocarcinoma.
    Article Snippet: .. After blocking with 5% skim milk, the membrane was probed overnight at 4 °C with the following primary antibodies: anti-CD81 (66866-1-Ig, Proteintech), anti-CD9 (25597-1-AP, Proteintech), anti-TSG101 (ab125011, Abcam), anti-HSP70 (10995-1-AP, Proteintech) and anti-Calnexin (ab22595, Abcam). .. Subsequently, the membrane was washed and incubated with HRP-conjugated secondary antibodies (SA00001-1 and SA00001-2, Proteintech) for 1 h at room temperature.



    Similar Products

    cd81 apc  (Miltenyi Biotec)
    95
    Miltenyi Biotec cd81 apc
    Cd81 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/CD81+Antibody%2C+anti-human%2C+REAfinity/pm42363190-83-56-58
    Average 95 stars, based on 1 article reviews
    cd81 apc - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    anti cd81  (Unchained Labs)
    86
    Unchained Labs anti cd81
    Anti Cd81, supplied by Unchained Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/anti+cd81/pmc13196426-3-5-10
    Average 86 stars, based on 1 article reviews
    anti cd81 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    anti cd81 pevio770  (Miltenyi Biotec)
    95
    Miltenyi Biotec anti cd81 pevio770
    Anti Cd81 Pevio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/CD81+Antibody%2C+anti-human%2C+REAfinity/pm42349165-112-37-40
    Average 95 stars, based on 1 article reviews
    anti cd81 pevio770 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    cd81  (Abmart Inc)
    86
    Abmart Inc cd81
    Cd81, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/anti+cd9/pm42286608-50-77-79
    Average 86 stars, based on 1 article reviews
    cd81 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    cd81 pe  (Miltenyi Biotec)
    95
    Miltenyi Biotec cd81 pe
    Cd81 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/CD81+Antibody%2C+anti-human%2C+REAfinity/pm42242979-264-43-46
    Average 95 stars, based on 1 article reviews
    cd81 pe - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    anti cd81 alexaflour647  (Oxford Nanoimaging Ltd)
    86
    Oxford Nanoimaging Ltd anti cd81 alexaflour647
    Anti Cd81 Alexaflour647, supplied by Oxford Nanoimaging Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/alexaflour647+anti+cd81/bio_rxiv__64898__2026__05__05__722048-141-23-24
    Average 86 stars, based on 1 article reviews
    anti cd81 alexaflour647 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    cd81  (Proteintech)
    96
    Proteintech cd81
    Cd81, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/CD81+Antibody/pmc13038339-74-45-50
    Average 96 stars, based on 1 article reviews
    cd81 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti cd81  (Boster Bio)
    91
    Boster Bio anti cd81
    NsPEFs engineering boosts the production of ADSCs-EVs with superior yield and stability A. Schematic illustration of the high-efficiency extraction of extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) using nanosecond pulsed electric fields (NsPEFs). B. Representative transmission electron microscopy (TEM) images of isolated Ctrl-ADSCs-EVs and NsPEFs-ADSCs-EVs, showing characteristic cup-shaped morphology and bilayer membrane (scale bars: 150 nm and 75 nm). C. Nanoparticle tracking analysis (NTA) showing the particle size distribution of EVs (n = 3). D. Western blot (WB) analysis confirming the positive expression of EV-specific markers <t>(CD81,</t> CD63, TSG101) and the absence of the negative markers (Calnexin, Histone H3, LaminA/C). Quantification is shown on the right (n = 3). E. The particle concentration of EVs. F. NsPEFs stimulation significantly enhanced both yield and protein output compared to Ctrl-ADSCs-EVs. G. Zeta potential measurement indicating colloidal stability (n = 3). H. Purity assessment expressed as the particle-to-protein ratio ( × 10 9 particles/μg). I. Viability of cells post-NsPEFs-ADSCs-EVs treatment assessed by trypan blue exclusion assay (scale bar: 1.7 mm). Data are presented as mean ± SEM from at least three independent experiments. Statistical significance was determined by unpaired two-tailed Student's t-test or one-way ANOVA with Tukey's post-hoc test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001; ns: not significant.
    Anti Cd81, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/Anti-CD81+Antibody/pmc12856188-111-66-67
    Average 91 stars, based on 1 article reviews
    anti cd81 - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    hamster  (Bio-Rad)
    93
    Bio-Rad hamster
    NsPEFs engineering boosts the production of ADSCs-EVs with superior yield and stability A. Schematic illustration of the high-efficiency extraction of extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) using nanosecond pulsed electric fields (NsPEFs). B. Representative transmission electron microscopy (TEM) images of isolated Ctrl-ADSCs-EVs and NsPEFs-ADSCs-EVs, showing characteristic cup-shaped morphology and bilayer membrane (scale bars: 150 nm and 75 nm). C. Nanoparticle tracking analysis (NTA) showing the particle size distribution of EVs (n = 3). D. Western blot (WB) analysis confirming the positive expression of EV-specific markers <t>(CD81,</t> CD63, TSG101) and the absence of the negative markers (Calnexin, Histone H3, LaminA/C). Quantification is shown on the right (n = 3). E. The particle concentration of EVs. F. NsPEFs stimulation significantly enhanced both yield and protein output compared to Ctrl-ADSCs-EVs. G. Zeta potential measurement indicating colloidal stability (n = 3). H. Purity assessment expressed as the particle-to-protein ratio ( × 10 9 particles/μg). I. Viability of cells post-NsPEFs-ADSCs-EVs treatment assessed by trypan blue exclusion assay (scale bar: 1.7 mm). Data are presented as mean ± SEM from at least three independent experiments. Statistical significance was determined by unpaired two-tailed Student's t-test or one-way ANOVA with Tukey's post-hoc test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001; ns: not significant.
    Hamster, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd81/Hamster+anti+Mouse+CD81/pmc12694724-2-2-4
    Average 93 stars, based on 1 article reviews
    hamster - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    NsPEFs engineering boosts the production of ADSCs-EVs with superior yield and stability A. Schematic illustration of the high-efficiency extraction of extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) using nanosecond pulsed electric fields (NsPEFs). B. Representative transmission electron microscopy (TEM) images of isolated Ctrl-ADSCs-EVs and NsPEFs-ADSCs-EVs, showing characteristic cup-shaped morphology and bilayer membrane (scale bars: 150 nm and 75 nm). C. Nanoparticle tracking analysis (NTA) showing the particle size distribution of EVs (n = 3). D. Western blot (WB) analysis confirming the positive expression of EV-specific markers (CD81, CD63, TSG101) and the absence of the negative markers (Calnexin, Histone H3, LaminA/C). Quantification is shown on the right (n = 3). E. The particle concentration of EVs. F. NsPEFs stimulation significantly enhanced both yield and protein output compared to Ctrl-ADSCs-EVs. G. Zeta potential measurement indicating colloidal stability (n = 3). H. Purity assessment expressed as the particle-to-protein ratio ( × 10 9 particles/μg). I. Viability of cells post-NsPEFs-ADSCs-EVs treatment assessed by trypan blue exclusion assay (scale bar: 1.7 mm). Data are presented as mean ± SEM from at least three independent experiments. Statistical significance was determined by unpaired two-tailed Student's t-test or one-way ANOVA with Tukey's post-hoc test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001; ns: not significant.

    Journal: Bioactive Materials

    Article Title: NsPEFs-enriched ADSCs-EVs alleviate osteoarthritis via RSPO3-mediated dual pro-chondrogenic and pro-M2 macrophage properties

    doi: 10.1016/j.bioactmat.2026.01.006

    Figure Lengend Snippet: NsPEFs engineering boosts the production of ADSCs-EVs with superior yield and stability A. Schematic illustration of the high-efficiency extraction of extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) using nanosecond pulsed electric fields (NsPEFs). B. Representative transmission electron microscopy (TEM) images of isolated Ctrl-ADSCs-EVs and NsPEFs-ADSCs-EVs, showing characteristic cup-shaped morphology and bilayer membrane (scale bars: 150 nm and 75 nm). C. Nanoparticle tracking analysis (NTA) showing the particle size distribution of EVs (n = 3). D. Western blot (WB) analysis confirming the positive expression of EV-specific markers (CD81, CD63, TSG101) and the absence of the negative markers (Calnexin, Histone H3, LaminA/C). Quantification is shown on the right (n = 3). E. The particle concentration of EVs. F. NsPEFs stimulation significantly enhanced both yield and protein output compared to Ctrl-ADSCs-EVs. G. Zeta potential measurement indicating colloidal stability (n = 3). H. Purity assessment expressed as the particle-to-protein ratio ( × 10 9 particles/μg). I. Viability of cells post-NsPEFs-ADSCs-EVs treatment assessed by trypan blue exclusion assay (scale bar: 1.7 mm). Data are presented as mean ± SEM from at least three independent experiments. Statistical significance was determined by unpaired two-tailed Student's t-test or one-way ANOVA with Tukey's post-hoc test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001; ns: not significant.

    Article Snippet: The antibodies used and the dilution ratios were as follows:Anti-INOS (1:800, Cohesion), Anti-Arginase 1 (1:800, BOSTER), Anti-LRP6 (1:800, BOSTER), Anti-Beta-catenin (1:800, BOSTER), Anti-CD163 (1:800, Abclonal), Anti-CD86 (1:800, BOSTER), Anti-LGR4 (1:800, Abclonal), Anti-IL-1β (1:800, BOSTER), Anti-IL-10 (1:1000, Bioss), Anti-MMP13 (1:800, BOSTER), Anti-COL2A1 (1:800, BOSTER), Anti-Histone H3 (1:1000, Nature Biosciences), Anti-Lamin A/C (1:1000, Nature Biosciences), Anti-Akt (1:1000, Nature Biosciences), Anti-pAkt (1:1000, Nature Biosciences), Anti-RSPO3 (1:1000, Abcam), Anti-CD63(1:800, BOSTER), Anti-CD81(1:800, BOSTER), Anti-TSG101(1:800, BOSTER), Anti-Calnexin(1:800, BOSTER).

    Techniques: Extraction, Derivative Assay, Transmission Assay, Electron Microscopy, Isolation, Membrane, Western Blot, Expressing, Concentration Assay, Zeta Potential Analyzer, Trypan Blue Exclusion Assay, Two Tailed Test